AP Biology · Unit 2 · Topic 2.9

Why Cells Have Rooms

Run the same reactions in one open space and in separate compartments, and the difference in what gets made is the answer.

Learning objectives 2.9.A Describe the membrane-bound structures of the eukaryotic cell. 2.9.B Explain how internal membranes and membrane-bound organelles contribute to compartmentalization of eukaryotic cell functions.

Watch it work · a two-minute demo for teachers

Narrated walkthrough of the “Run the experiment” tab. Captions are on by default.

Two reactions, one substrate

A cell holds a pool of substrate. One enzyme turns it into the product the cell needs; a second enzyme destroys it. Both enzymes are equally fast. The only thing you can change is how much of the substrate is shut away with the wanted enzyme.

0%
substrate left product — the reaction the cell wants waste — the competing reaction
Product0
Waste0
Substrate left0
Against an open cytosol—
Move the slider from 0% to 100% and watch the bar on the right: the waste band shrinks and the product band grows to take its place.

The result, plotted

Product against the fraction of substrate enclosed. Nothing else changes: same substrate, same enzymes, same time.

product waste

Why this is a fair comparison

At 0% enclosed, the compartment model behaves exactly like an open cytosol — the two runs are identical. That is what makes the comparison mean something: the only difference between 0% and 100% is the compartment.